Id-specific T-cell proliferation. PBMNC were isolated preimmunization and after each immunization and were cultured for 6 days in medium alone (med.) or medium containing Id at 0.1, 1.0, 10, and 100 μg/mL (0.1, 1.0, 10, and 100 respectively). Overnight3H thymidine incorporation into DNA was determined on day 6. (A) T-cell proliferation assay of patient 1 before any immunization (prevaccine) and 3 months post-Id/KLH vaccine no. 5. Proliferation in medium containing an irrelevant isotype-matched Ig at 100 μg/mL was statistically significant less (P = .014) with 3,296 ± 445 cpm. Comparable results were obtained in experiments performed 1 and 2 months post-Id/KLH vaccine no. 5. (B) T-cell proliferation assay of patient 9 before any immunization (prevaccine) and after Id/KLH vaccine no. 1. Proliferation in medium with an irrelevant isotype matched Ig at 100 μg/mL was at 519 ± 93 cpm (P = .05). Id-specific proliferation was also seen after Id/KLH vaccine nos. 2 and 3. (C) T-cell proliferation of patient 1 in response to the immunologic carrier KLH at 3 months post Id/KLH vaccine no. 5. (D) T-cell proliferation of patient 9 in response to the immunologic carrier KLH after Id/KLH vaccine no. 1. The assays were run in triplicate or quadruplicate and mean values are depicted. Error bars denote one standard deviation. Using the Mann-Whitney U test, prevaccine and postvaccine Id-specific responses were significantly different at all Id concentrations studied (P = .014 patient 1,P = .05 patient 9).
