Correlation of eotaxin mRNA expression and grade of tissue eosinophilia in HD. Tissue eosinophilia was quantified by counting the mean number of eosinophils/high powered field (at least 20 high powered fields were examined in each specimen). Tissue samples were classified as having 0 eosinophils/high powered field; 1 to 25 eosinophils/high powered field; or >25 eosinophils/high powered field.