Immunohistochemical analysis of IP-10, Mig, and eotaxin protein expression in HD tissues: MC, NS, and NLP, and lymphoid hyperplasia. Paraffin-embedded tissue sections were stained with hematoxylin and eosin (H&E), an anti-IP–10 heteroantiserum (IP-10), an anti-Mig heteroantiserum (Mig), and an antieotaxin heteroantiserum (eotaxin). Primary antibodies were detected with biotinylated antirabbit IgG, followed by streptavidin-peroxidase complexes.