Fig. 6.
![Fig. 6. NF-κB binding activity in various cell lines. Nuclear extracts were prepared from cells. Nuclear extracts (5 μg) were incubated with a [32P]-radiolabeled oligonucleotide that corresponds to the κB sequence of the IL-2R gene and analyzed by EMSA. (B) In super-shift assays, antibody specific for each NF-κB subunit (indicated above the lanes) was incubated with MT-2 and TL-Om1 extracts before probe addition.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/93/7/10.1182_blood.v93.7.2360/5/blod4071006abw.jpeg?Expires=1724260030&Signature=aBjEkX-mdJxDJkqLKw6z~wgE~dujl81xtd5x8lkV7-9e7ZNbPnQAMS1nBh2rQtbGHWtYZEn31SrFDl6Md90wj13MCCnV52lKU2JIetFGWXmErVTHIFaoZUEyR2mx5iioTRkd5shXc1cruCyMw0VGLdJCAFYxrHiYlfw0~EIGay1GhpcbVOtJizdNJDNewY1g7A3D1DfxFGJpULGQnO7SjPwPrrAjAjyit5a9O~ed66mmDrpKTKYZj9fc5acR5E~GKHblqyGMkacjENYc~M403LhI29JVDQoIjgAvgggphrMsTqtG2F8--xSjHppdfV~wj0guD3FvDGMxQIR0tBUsFg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
NF-κB binding activity in various cell lines. Nuclear extracts were prepared from cells. Nuclear extracts (5 μg) were incubated with a [32P]-radiolabeled oligonucleotide that corresponds to the κB sequence of the IL-2R gene and analyzed by EMSA. (B) In super-shift assays, antibody specific for each NF-κB subunit (indicated above the lanes) was incubated with MT-2 and TL-Om1 extracts before probe addition.
