Fig. 7.
![Fig. 7. Effect of 1,25(OH)2D3 on the levels of CKIs (A), cyclins (B), and CDKs (C) in MCF-7 cells. Whole cell extracts isolated from cells grown in the absence or presence of 1,25(OH)2D3 (1 × 10−7 mol/L) for 0 to 48 hours were analyzed by Western blot. Twenty micrograms of whole cell extract was used in each lane and probed with antibodies to the indicated proteins. As a control, cells were grown for up tp 48 hours in the absence of ligand (ethanol alone), and p21 levels were determined (shown in [A]).](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/93/8/10.1182_blood.v93.8.2721/5/blod40828007w.jpeg?Expires=1724269804&Signature=qrYzib5ciGoLaiUJweQFMBMIbEp6fgkk90w0FULpcn61Lv40-23rKdSYQsIQgxA06Aqxpv4OjNBpW7R5IkK-mEqhqJqcj6FHdOwvYbh2gkP3aPm0FWcQTSWwwF6KDAYAhqR6AyKCkRXwYMerue8W582hi8dE8tU4GduZ2XgL00oNbbZaDcmSwqA0Em-RFzjXH850cb0AX7QHcrdvCDmDr4PDEPH0JMG6yKqccudMn5rIMGBcSm5RB~b4lBqDBBal2pIsNeJbuAAsXGETWM~P5apOEPq1TDrq3BOrpPmgvLywVfrPddRcBXFwH~eXnDIQIISXuLvBmlhnNxGt~Qw0~w__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Effect of 1,25(OH)2D3 on the levels of CKIs (A), cyclins (B), and CDKs (C) in MCF-7 cells. Whole cell extracts isolated from cells grown in the absence or presence of 1,25(OH)2D3 (1 × 10−7 mol/L) for 0 to 48 hours were analyzed by Western blot. Twenty micrograms of whole cell extract was used in each lane and probed with antibodies to the indicated proteins. As a control, cells were grown for up tp 48 hours in the absence of ligand (ethanol alone), and p21 levels were determined (shown in [A]).
