RT-PCR analysis of BCR/ABL and C-ABL transcripts in Dami/HEL, Meg-01, and K562 leukemic cell lines. (A) C-ABL expression was analyzed by RT-PCR using 1 μg total RNA from K562 (lane 1), Mo7e (lane 3), Dami/HEL (lane 4), and Meg-01 (lane 5) cell lines. RT-PCR was performed without cellular RNA as a negative control (lane 2). (B) BCR/ABL expression was analyzed by RT-PCR using 0.01 μg or 1 μg total RNA from K562 cells as positive controls (lanes 1 and 2), or using 1 μg of total RNA from Mo7e cells treated without (lane 3) or with 100 ng/mL TPO (lane 4), from Dami/HEL cells treated without (lane 5) or with 100 ng/mL TPO (lane 6), and from Meg-01 cells treated without (lane 7) or with 100 ng/mL TPO (lane 8). The sizes of PCR products are indicated in base pairs.