Fig. 3.
![Fig. 3. Effects of cytokines on activation of a STAT-like DNA-binding factor in Mo7e cells. Nuclear extracts from Mo7e cells grown in the absence of cytokine (CTL), or in the presence of 40 ng/mL EPO, 400 ng/mL TPO, 40 ng/mL GM-CSF or 40 ng/mL IL-3, were incubated with [32P]-labeled IRF-1 GAS oligonucleotide. The STAT-like DNA-binding complex and unbound probe were separated on 5% native polyacrylamide gel. The autoradiograph shows the DBF, NS, and free probes (P).](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/93/7/10.1182_blood.v93.7.2369/5/blod40718003w.jpeg?Expires=1724269446&Signature=FxHx2ZzdQhrG6lgYcQyDVgumDqfvQpqc8XOhhugOhHmqeRB~UXeVvwvXI2ytxJYDNh4CFE5n9HR0dYAzKR47NJYdRl-CEz~BeGi-SdFJVepqqntVgiDB0tUBJgFvufe1rVTUC0AqgejcA9NLf7kZ6hSBkiDvTnFFg6pT8g3ylqp8~YIVkmndQYjc0I0nieiJemV5sSNJO7C7TlcHxYvSKpBX9ZYxYxhplR7r8Er3Gyk3qx5nHYdcM76y1Ze1GDUPkzd0RxONZPurLJ5wrcmDyQ3E-HfDKF4RYR8fOJE-BVmbBii-txZNmo5Wcb9iDrh3-EH1R2IjWEMwClO7ulcOkQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Effects of cytokines on activation of a STAT-like DNA-binding factor in Mo7e cells. Nuclear extracts from Mo7e cells grown in the absence of cytokine (CTL), or in the presence of 40 ng/mL EPO, 400 ng/mL TPO, 40 ng/mL GM-CSF or 40 ng/mL IL-3, were incubated with [32P]-labeled IRF-1 GAS oligonucleotide. The STAT-like DNA-binding complex and unbound probe were separated on 5% native polyacrylamide gel. The autoradiograph shows the DBF, NS, and free probes (P).
