Fig. 2.
![Fig. 2. Constitutive activation of a STAT-like DNA-binding factor in Dami/HEL and Meg-01 cells. Nuclear extracts from each cell line cultured in the absence of cytokine (CTL) or in the presence of as much as 400 ng/mL TPO or 40 ng/mL IL-3, were incubated with [32P]-labeled, double-stranded IRF-1 GAS oligonucleotide. The DNA-binding complex and unbound probes were separated electrophoretically on 5% nondenaturing polyacrylamide gels. The autoradiograph shows the STAT-like DNA-binding factor (DBF) and the nonspecific bands (NS).](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/93/7/10.1182_blood.v93.7.2369/5/blod40718002w.jpeg?Expires=1724268558&Signature=QoCsQJwBqHLE~EmjAichDCaLmCPev~NPL1zWS1tzXpTiPlgu-ZLwPeoZqGk8vJ0BobxLL5lvQRkfQP33UPBjSBEmcQR000pg50OhJTCTrTzIFU158dSb9HCtYEx~jYvEPnSCmAm6UCyQYbhhIkzKhvtrcjTAZNNTPv6iiwoFoet11WcdQZcQOXT-etyMtmeJpPIWiBMqcEk7mKqn5WG~qWhWgTzLHn7xCWUJ7L1z1B77mozwLhjI2adWs0U~WNTUNlfBMJOV8klJmUEKsU2ucZem-P5Q8SkEtrvg6SOIeQ32tW1BHzBtFZlaKujA~H2s3G6Uci060uphES2VeY8vEw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Constitutive activation of a STAT-like DNA-binding factor in Dami/HEL and Meg-01 cells. Nuclear extracts from each cell line cultured in the absence of cytokine (CTL) or in the presence of as much as 400 ng/mL TPO or 40 ng/mL IL-3, were incubated with [32P]-labeled, double-stranded IRF-1 GAS oligonucleotide. The DNA-binding complex and unbound probes were separated electrophoretically on 5% nondenaturing polyacrylamide gels. The autoradiograph shows the STAT-like DNA-binding factor (DBF) and the nonspecific bands (NS).
