Chromosomal localization of clone 59-2 by hybrid panel mapping. Sequence-specific primers were used in a PCR reaction with DNA of hybrid cell lines. In the first PCR reaction, DNA of multichromosomal somatic cell hybrids with several human chromosomes in a rodent chromosome background was amplified in the PCR reaction (top part of the figure). Finally the exact localization was determined using DNA of monochromosomal somatic cell hybrids with one or several human chromosomes in a rodent chromosome background (bottom part of the figure). Clone 59-2 was mapped to chromosome 6. (Top) VI, DNA marker; lane 1, NA09927; lane 2, NA09929; lane 3, NA 09931; lane 4, NA09935A; lane 5, NA00347A (hamster DNA); lane 6, NAIMR91 (human genomic DNA); lane 7, human blood DNA; lane 8, no DNA. (Bottom) VI, DNA marker; lane 1, NA10253 (chromosome 3); lane 2, NA10115 (chromosome 4); lane 3, NA10629 (chromosome 6); lane 4, NA00347A (hamster DNA); lane 5, NAIMR91 (human genomic DNA); lane 6, human blood DNA; lane 7, no DNA.