Development of Mk generation from CB CD34⁺cells by sIL-6R, IL-6, and SCF. (A) A typical Mk generated in serum-free suspension culture was positively immunostained with anti-IIbIIIa MoAb by APAAP technique. Magnification: ×1000. (B) A representative Mk colony derived from CD34⁺ cells in serum-free methylcellulose culture (original magnification ×100).