Fig. 7.
Fig. 7. Time-dependent effects of either 1,25(OH)2D3 alone or in combination with RA on levels of p27KIP1 mRNA in UF-1 cells. Cells were cultured for the indicated durations with either 10−7mol/L 1,25(OH)2D3 alone or in combination with 10−7 mol/L RA. Total RNA (15 μg per lane) was blotted and hybridized with a p27KIP1 cDNA probe. Bottom panel shows the ethidium bromide–stained gel with 28S and 18S ribosomal RNA demonstrating equivalent RNA-loading per lane. Expression of p27KIP1 transcripts relative to the expression in control cells was determined by densitometry.

Time-dependent effects of either 1,25(OH)2D3 alone or in combination with RA on levels of p27KIP1 mRNA in UF-1 cells. Cells were cultured for the indicated durations with either 10−7mol/L 1,25(OH)2D3 alone or in combination with 10−7 mol/L RA. Total RNA (15 μg per lane) was blotted and hybridized with a p27KIP1 cDNA probe. Bottom panel shows the ethidium bromide–stained gel with 28S and 18S ribosomal RNA demonstrating equivalent RNA-loading per lane. Expression of p27KIP1 transcripts relative to the expression in control cells was determined by densitometry.

Close Modal
This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal