Fig. 5.
![Fig. 5. Expression of p21WAF1/CIP1 mRNA in HL-60 and NB4 cells, and p21WAF1/CIP1 and p27KIP1 transcripts in UF-1 cells. Cells were cultured with either 10−7 mol/L 1,25(OH)2D3 (A) or 10−7 mol/L all-trans RA (B) for 48 hours. Total RNA (15 μg per lane) was extracted and analyzed by Northern blot with [32P]-labeled p21WAF1/CIP1 or p27KIP1 cDNA probe. Bottom panel shows the ethidium bromide–stained formaldehyde gel before Northern blotting; the levels of 28S and 18S ribosomal RNA are comparable in each lane.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/93/7/10.1182_blood.v93.7.2225/5/blod40705005w.jpeg?Expires=1724160165&Signature=f3-a4nbCLufJM2PAfq4UiCl0a~w4JimA19075czBYXCHWKzaBTqmxGpqjQeyXdMzdD6i4MvrZmw4SccD~E8SGhylmnziHBSJusrqSjc8m7RWIksJne0Qe0nlqTO7W~s1AsbKqYI39YW5IL~hhgZWmGby8BoT07pmJoNclJraBLGADsbwEoCV3gfH~eEWaCDfbNquPXbUk8mZ78ft8uREXlih6c6Rt1oqe2DILI-GLthgGh~WCjN6iNjHmVHiTFGrwAZtwC4-wGff0jVe6WdBmYWd9x8XJiJcmkmPZCyP4oMAEQ4iNUgGsBlHEFd7y2oPJHzr~~I3-vMsleQfnnh4VQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Expression of p21WAF1/CIP1 mRNA in HL-60 and NB4 cells, and p21WAF1/CIP1 and p27KIP1 transcripts in UF-1 cells. Cells were cultured with either 10−7 mol/L 1,25(OH)2D3 (A) or 10−7 mol/L all-trans RA (B) for 48 hours. Total RNA (15 μg per lane) was extracted and analyzed by Northern blot with [32P]-labeled p21WAF1/CIP1 or p27KIP1 cDNA probe. Bottom panel shows the ethidium bromide–stained formaldehyde gel before Northern blotting; the levels of 28S and 18S ribosomal RNA are comparable in each lane.
