MNC PCA. MNC were isolated from 6 healthy donors at baseline (T0), after 5 days of rHuG-CSF administration (T2), and 1 week after stopping the drug (T3). The dashed line indicates the mean PCA of MNC from the normal control subject group. The PCA of lysed MNC suspensions was tested after 4 hours of incubation with 1 μg/mL LPS (+LPS, right panel) or the vehicle (−LPS, left panel) by the one-stage recalcification assay of normal human plasma. Results are expressed as arbitrary units obtained from a calibration curve of standard thromboplastin (RBT, from 10−1 to 10−6); 1 unit equals the activity of 1 mEq/mL RBT in the one-stage clotting assay. PCA was identified as TF, as indicated in Materials and Methods. Statistical analysis as in Fig 1. *P < 0.05 v T0.
