Changes of PMN activation parameters in healthy HCP donors before (T0), during (T1 and T2), and 1 week after stopping (T3) rHuG-CSF administration. (A) Flow cytometry analysis of the expression of CD11b/CD18 on PMN cell surface; results are expressed as the mean intensity fluorescence (MIF). (B) Plasma concentration of elastase-1AT complex during rHuG-CSF administration. (C) Elastase activity of lysed PMN isolated from citrated whole blood. The results are the mean ± SEM. Dashed lines represent the cut-off of normal control values (mean + 2SD). Statistical analysis by ANOVA for repeated measures and Fisher’s test for multiple comparisons is shown. *P < .05, **P < .01 v T0.