Number of human CFCs harvested from transplanted animals is lower in animals that received cultured cells versus noncultured cells. Circles represent individual mice that received noncultured cells (day 0) or cells that were cultured for 1, 2, or 3 days in serum-free medium supplemented with SF, FL, and IL-3. The total number of human colonies per femur was calculated by dividing the number of total cells obtained per femur by the number of cells plated per methylcellulose dish (usually 2 × 105 cells), multiplied by the average number of colonies scored in a duplicate set of dishes. There were no significant differences in the relative contribution of the types of colonies between cultured and noncultured cells. Shown are the combined data from three experiments.
