Binding of +-MITF to the CATGTG motif in 5′ flanking region of the MMCP-4 gene. (A) EGMSA using GST-+-MITF and GST-mi-MITF fusion proteins. The 5′-GTCCTCTCCTCCATGTGCTCATA oligonucleotide containing a CATGTG motif (probe 1, nt -394 to -372; numbers refer to the sequence shown in Fig 5) and the 5′-CACTACAGATGTCTGTGTTTGTG-3′ oligonucleotide containing a CAGATG motif (probe 2, nt -371 to -349) were used (CANNTG motifs are boxed). The DNA-protein complex is indicated by an arrowhead. (B) Competitive DNA binding assay with GST-+-MITF. Two competitors were synthesized. The oligo 1 was identical to the probe 1; oligo 3 had the mutation at the CATGTG motif (to CTTGAG). DNA-protein complexes are indicated by an arrowhead.