SHIP deletion. (A) The two bands produced using the 2666-3247 primer set, shown in Fig 1B, were subcloned and sequenced. The larger (581 bp) band corresponds to the published SHIP sequence. The smaller (398 bp) band has a deletion of 183 nucleotides at the site shown, but is otherwise identical. (B) SHIP genomic DNA was isolated from a lambda genomic DNA library. Phage clones which hybridized with the 581 bp product of the 2666-3247 PCR primers were sequenced to show the NPXY-containing exon. Exon sequence is in upper case letters, and the sequence of the ▵183 deletion is in bold type. The verified splice acceptor and donor are shown in shaded boxes, and the potential splice sites that would produce the ▵183 deletion as an intron are shown in open boxes. Numbering refers to the SHIP cDNA sequence as in Fig 1.