SHIP RT-PCR. (A) The 3,570 nucleotide ORF of murine SHIP cDNA is shown with structural and potential functional domains labeled. The NPXY and YIGM motifs are depicted (▪), as well as the proline-rich potential SH3 domain binding regions (□). Also shown are the binding sites for the PCR primer sets used. Nucleotide numbering refers to the cDNA sequence of murine SHIP, Genbank accession numberU51742. (B) RT-PCR was conducted using RNA from the WEHI-3 and FDC-P1 myeloid cell lines and the primer sets shown in Fig 1A. The products were separated by electrophoresis in a 1% agarose gel and stained with ethidium bromide. Amplification of cDNA with primers to GAPDH was included to verify cDNA quality.