Expression of Cyp11A in the spleen. Total RNAs were prepared from the spleens of wild-type adult (Adult), and wild-type (Nor) and mFtz-F1 gene–disrupted (KO) new born (B0) mice. cDNAs reverse-transcribed from the RNAs were used for PCR with specific primers for Ad4BP/SF-1 (A), Cyp11A (B), and β-actin (C) as described in Materials and Methods. Arrows indicate the expected length of PCR products.