Fig. 3.
In vitro function of mouse platelets. (A) ADP-induced platelet aggregation. Citrated PRP was prepared from VN +/+ mice (n = 2) and VN −/− mice (n = 2). After adjusting platelet counts to 2.5 × 108/mL, ADP (12.5 μmol/L)-induced platelet aggregation was studied in 96-well microtiter plates that were warmed to 37°C and automatically shaken. (B) Thrombin-induced platelet aggregation. Washed platelets were prepared from VN +/+ mice (n = 3) and VN−/− mice (n = 3) and suspended in Tyrode’s buffer at a concentration of 2.5 × 108/mL. Thrombin (1 U/mL)-induced platelet aggregation was studied as described for ADP. Data points represent the mean of triplicate experiments ± 1 SD.
