Fig. 6.
Effect of leptin on proliferation of the OCI/AML2 (⧫) and MO7E (▩) cell line. MO7E cells were growth-factor starved for 18 hours. OCI/AML2 or MO7E cells were adjusted to the starting concentration of 100,000 cells/mL and cultured with increasing concentrations of rH leptin. After 48 hours of incubation, cultures were pulsed for 12 hours with [3H]thymidine (0.1 μCi/well), harvested onto filter paper, immersed in scintillation fluid, and quantitated in counts per minute. Each data point was sampled in triplicate. Data from one of two experiments that yielded similar results are shown.

Effect of leptin on proliferation of the OCI/AML2 (⧫) and MO7E (▩) cell line. MO7E cells were growth-factor starved for 18 hours. OCI/AML2 or MO7E cells were adjusted to the starting concentration of 100,000 cells/mL and cultured with increasing concentrations of rH leptin. After 48 hours of incubation, cultures were pulsed for 12 hours with [3H]thymidine (0.1 μCi/well), harvested onto filter paper, immersed in scintillation fluid, and quantitated in counts per minute. Each data point was sampled in triplicate. Data from one of two experiments that yielded similar results are shown.

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