Fig. 8.
IFN-–induced STAT5 DNA binding to the IFP53GAS element. T cells were stimulated with IFN- or IL-2 for 30 minutes, and nuclear extracts were prepared and incubated with anti-STAT1 or anti-STAT5 antibodies for 1 hour on ice, followed by EMSA with 32P-labeled IFP53 GAS probe.
