Fig. 1.
Schematic of the HIV-1 Vpr transduction vector. pSLX-CMV-Vpr encodes a Vpr expression cassette from HIV-1 (strain NL4-3) downstream of the cytomegalovirus (CMV) promoter (pCMV). The retroviral packaging cell-line, PA317, provides, in trans, viral proteins necessary for vector transfer.30 Below, the linear map of Vpr, 96 amino acids, denotes the approximate location of domains important for Vpr function.31,32
