Fig. 10.
Measurement of lyp1 phosphatase activity. Anti-lyp immunoprecipitates from untransfected and pcDNA3-lyp1–transfected cells were prepared in pervanadate-free lysis buffer and incubated with33P-labeled substrate Raytide. At the indicated time points, reactions were stopped by the addition of charcoal and the free33P released from the peptide and now present in the supernatant was measured by liquid scintillation counting.
