Localization of Lyp1 and Lyp2 in transiently transfected COS-7 cells by immunofluorescence. COS-7 cells were transiently transfected with HA-tagged LyP1 and LyP2 cDNAs in pcDNA3 and immunofluorescent detection was performed with a monoclonal antibody against the HA tag (original magnification × 1,000). (A) Cells transfected with HA-Lyp2 cDNA. (B) Cells transfected with HA-LyP1 cDNA.