Fig. 5. Expression profile of Lyp1 and Lyp2 transcripts. (A) Two micrograms of poly A+ RNA from various human tissues and cell lines (OCI/AML3, acute myeloblastic leukemia cell line; K562, erythroleukemia cell line; and U937, monocytes cell line) were hybridized with a 1.3-kb cDNA probe common to both Lyp1 and Lyp2 (exposure time, 7 days) and with actin (exposure time, 24 hours). (B) RNA from immune relevant human tissues (Clontech) was blotted first with a cDNA probe from the unique 280-bp 3′ nucleotides sequence of Lyp2 (including the untranslated sequence) and then, after stripping, with a 600-bp cDNA probe from the unique 3′ nucleotide sequence of Lyp1 (exposure time, 7 days) and with actin (exposure time, 24 hours). The sizes of the RNA markers are indicated in kilobases.
Fig. 5.

Expression profile of Lyp1 and Lyp2 transcripts. (A) Two micrograms of poly A+ RNA from various human tissues and cell lines (OCI/AML3, acute myeloblastic leukemia cell line; K562, erythroleukemia cell line; and U937, monocytes cell line) were hybridized with a 1.3-kb cDNA probe common to both Lyp1 and Lyp2 (exposure time, 7 days) and with actin (exposure time, 24 hours). (B) RNA from immune relevant human tissues (Clontech) was blotted first with a cDNA probe from the unique 280-bp 3′ nucleotides sequence of Lyp2 (including the untranslated sequence) and then, after stripping, with a 600-bp cDNA probe from the unique 3′ nucleotide sequence of Lyp1 (exposure time, 7 days) and with actin (exposure time, 24 hours). The sizes of the RNA markers are indicated in kilobases.

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal