Fig. 5.
![Fig. 5. PI-6 coimmunoprecipitates with cathepsin G from U937 cells as a 56-kD complex. (A) U937 cells were [35S]methionine labeled, lysed, and immunoprecipitated with rabbit polyclonal antibodies recognizing cathepsin G, proteinase 3, or human leukocyte elastase. Immune complexes were separated by 12.5% SDS-PAGE under reducing conditions. (B) U937 cells were lysed and incubated at 37°C for 10 minutes. Extracts were immunoprecipitated with rabbit polyclonal antibodies recognizing cathepsin G, proteinase 3, or human leukocyte elastase. Immune complexes were separated by 12.5% SDS-PAGE and separated proteins were immunoblotted using monoclonal 3A anti–PI-6 antibody.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/93/6/10.1182_blood.v93.6.2089.406k10_2089_2097/5/blod40610005aw.jpeg?Expires=1724240164&Signature=P64jALh4wZQ2IXyBHVfksC~Zwfx9EBWGin0Fq5QUowWWJBqViMV52YlPv4zaktTKfqk4t1hMvlKUuiWMchG6YJoFZVFUB1jk-uPkr1hwmCMAhQ1xNTEgJS3EXdaavK0yd6kbHa0VG9MYEnMB84szPRe6N1AH2vrDFXPi5YZPLFgPlUsSEZDxI1PhOVrUhz4fbfrYqCX6N7j3G5k1bH29YgrdmcIw5oO~g7wRYCIK~WMNZwwleAVIaR2X-bMQif2D9eNTbNyQpHzkeVjfNwDsfIrePJ800KZY6eLznyxc9N4Fus6FEl0Y8kSn-QjGl59LRZthILr8rsAUgWtAdcJj3Q__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
PI-6 coimmunoprecipitates with cathepsin G from U937 cells as a 56-kD complex. (A) U937 cells were [35S]methionine labeled, lysed, and immunoprecipitated with rabbit polyclonal antibodies recognizing cathepsin G, proteinase 3, or human leukocyte elastase. Immune complexes were separated by 12.5% SDS-PAGE under reducing conditions. (B) U937 cells were lysed and incubated at 37°C for 10 minutes. Extracts were immunoprecipitated with rabbit polyclonal antibodies recognizing cathepsin G, proteinase 3, or human leukocyte elastase. Immune complexes were separated by 12.5% SDS-PAGE and separated proteins were immunoblotted using monoclonal 3A anti–PI-6 antibody.
