Comparison of primer efficiency. (A) 100 pg, 10 pg, and 1 pg of RHAMM^FL and RHAMM⁻⁴⁸ were amplified with primer set 1. The amplification products for RHAMM^FL(666 bp) and RHAMM⁻⁴⁸ (613 bp) were analyzed by gel electrophoresis and visualized by ethidium bromide staining. (B and C) 100 pg,10 pg, and 1 pg of RHAMM^FL and RHAMM⁻¹⁴⁷ were amplified with primer set 3. The amplification products for RHAMM^FL (989 bp) and RHAMM⁻¹⁴⁷ (842 bp) were transferred to a nylon membrane and hybridized to a DIG-labeled RHAMM probe. RHAMM was detected by chemiluminescence; exposure was for 1 hour in (B) and for 10 hours in (C).