Fig. 6.
Fig. 6. A NOS inhibitor partially reverses the HIV-1 suppressive effect of astrocytes. MDM precultured for 7 days in DMEM medium were infected with HIV-1ADA. At day 1 postinfection, infected MDM were cocultured with HAA at a 2:3 ratio. Cocultures were fed with fresh DMEM medium with or without L-NMMA (500 μmol/L) every 3 days. Data are representative of two experiments and reflect the average levels of RT activity present in duplicate samples of supernatants, which differed by not more than 15%.

A NOS inhibitor partially reverses the HIV-1 suppressive effect of astrocytes. MDM precultured for 7 days in DMEM medium were infected with HIV-1ADA. At day 1 postinfection, infected MDM were cocultured with HAA at a 2:3 ratio. Cocultures were fed with fresh DMEM medium with or without L-NMMA (500 μmol/L) every 3 days. Data are representative of two experiments and reflect the average levels of RT activity present in duplicate samples of supernatants, which differed by not more than 15%.

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