Fig. 2.
Cells producing iNOS in cocultures are astrocytes but not MDM. MDM precultured for 7 days in DMEM medium were infected with HIV-1ADA. At day 1 postinfection, infected MDM were cocultured with HFA and were fed with fresh DMEM medium every 3 days. At day 21 postinfection, cells were stained with antibodies for CD14, GFAP, and iNOS using an intracellular antigen detection method and analyzed using a flow cytometer.
