Fig. 9.
![Fig. 9. Phage bearing a specific PMN-binding peptide sequence induce a pertussis toxin-sensitive calcium signal in PMN. (A) As described in Materials and Methods, INDO-1 loaded PMN were suspended for 2 minute at 37°C in a Ca++-containing buffer and cytosolic [Ca++] measured by spectrofluorimetry before and after the addition of 4 × 1010 of phage bearing the sequences indicated. (B) PMN were treated for 2 hours with pertussis toxin or buffer (control), loaded with INDO-1 and treated with FGDNLTGRW-displaying phage as in (A). Positive and negative controls, respectively (not shown), included stimulation with 10−7 mol/L fMLF and immune complexes.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/93/5/10.1182_blood.v93.5.1738/4/blod40518009x.jpeg?Expires=1724242863&Signature=3JBOzb1UlUmdAUwn0WqKkKIDaOdx7tx5YcNEM6HJlpD8swREJreuCFNV3ZLBxdkZymEXOtmrHlwDfIycQpC16uxNV9BlGrgWcMZ8zPxgmYnA69dkBVxQ-L8f6Yc9BcWW6DUJ3laa5J9--qCoYgU1WvdOKTgk~BPfUnE0OZUUEuW1Gk5Fts1y5vAvY4mvj-YjJHnw99JVn8KA0tQ-ZadXykBsEo9W3jFVwK9YXfPyD8C68QvCbGhr1lanJQaXD0EaRPi7cXKQR0ULSYNUyfzdCXpHB8Z4qqjVFwWjTK19C9GacV-LHpVgppjLTYtM7Vq5SwctmP8XUHoDTp6f9XFFRg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Phage bearing a specific PMN-binding peptide sequence induce a pertussis toxin-sensitive calcium signal in PMN. (A) As described in Materials and Methods, INDO-1 loaded PMN were suspended for 2 minute at 37°C in a Ca++-containing buffer and cytosolic [Ca++] measured by spectrofluorimetry before and after the addition of 4 × 1010 of phage bearing the sequences indicated. (B) PMN were treated for 2 hours with pertussis toxin or buffer (control), loaded with INDO-1 and treated with FGDNLTGRW-displaying phage as in (A). Positive and negative controls, respectively (not shown), included stimulation with 10−7 mol/L fMLF and immune complexes.
