Fig. 2.
Fig. 2. Neutrophil-binding phage peptide sequences isolated from the linear (X9) phage display library. The unique region of phage DNA recovered from the third round of affinity purification was sequenced and the motifs aligned to form the consensus DLXTSK(M/L)X(V/I/L) and GPNLTGRW, respectively. Identical residues and conservative substitutions are shown in bold. Following sets are considered to contain homologous amino acids (I, L, V), (K, R), (D, E), (S, T), (Y, F). The number of clones encoding the same peptide is shown in parentheses. The linker sequence at the amino terminus (GPP, not shown) was correctly displayed in all clones. Abbreviations for the amino acid residues are as follows: A, Ala; C, Cys; D, Asp; E, Glu; F, Phe; G, Gly; H, His; I, Ile; K, Lys; L, Leu; M, Met; N, Asn; P, Pro; Q, Gln; R, Arg; S, Ser; T, Thr; V, Val; W, Trp; and Y, Tyr.

Neutrophil-binding phage peptide sequences isolated from the linear (X9) phage display library. The unique region of phage DNA recovered from the third round of affinity purification was sequenced and the motifs aligned to form the consensus DLXTSK(M/L)X(V/I/L) and GPNLTGRW, respectively. Identical residues and conservative substitutions are shown in bold. Following sets are considered to contain homologous amino acids (I, L, V), (K, R), (D, E), (S, T), (Y, F). The number of clones encoding the same peptide is shown in parentheses. The linker sequence at the amino terminus (GPP, not shown) was correctly displayed in all clones. Abbreviations for the amino acid residues are as follows: A, Ala; C, Cys; D, Asp; E, Glu; F, Phe; G, Gly; H, His; I, Ile; K, Lys; L, Leu; M, Met; N, Asn; P, Pro; Q, Gln; R, Arg; S, Ser; T, Thr; V, Val; W, Trp; and Y, Tyr.

Close Modal

or Create an Account

Close Modal
Close Modal