Modulation of tie-1, but not E-selectin, cell surface expression by BB-24. HUVECs were incubated in the presence or absence of 5 μmol/L BB-24 for 10 minutes before the addition of TNF (green) or DMSO (blue) for 1 hour. After harvesting with versine-EDTA, cells were stained on ice with Abs specific for tie-1 or CD62E (E-selectin). Control samples were stained with isotype matched Abs (black). FACS analysis was performed as described in Materials and Methods.