Detection of KSHV gene sequences in BMDCs and PBDCs from MM patients and normal donors. DNA isolated from BMDCs from 15 MM patients and 6 normal donors was assayed for KSHV gene sequences by PCR using primers that amplify KS330₂₃₃, followed by a nested PCR using primers internal to this sequence to yield a final PCR product of 186 bp. Results from 10 MM patient BMDCs (lanes 2 through 11) and 3 normal donor BMDCs (lanes 12 through 14) are shown in (A). (B) Results of PCR on PBDCs DNA from 5 MM patients (lanes 2 through 6) and 4 normal donors (lanes 7 through 10). In each case DNA from BCBL-1 cell line (lane 1) served as the positive control. β-Actin was amplified in all samples to ensure adequacy and integrity of DNA.