American Society of Hematology

Fig. 2.

$Fig. 2. Immunoblot analyses of neutrophils from CGD patients, T.M. (A), T.H. (B), and J.O. (C). The membrane fractions from neutrophils (1 to 2 × 106 cell equivalents) were electrophoresed and transferred to nitrocellulose membranes, which were treated with antiserum against polypeptides of p22-phox and gp91-phox and stained with Ponceau. (A) Patient T.M. (3), mother (2), and controls (1 and 4). (B) Patient T.H. (2) and controls (1 and 3). (C) Patient J.O. (2) and controls (1 and 3). The protein concentration of the variant CGD (B) loaded was higher than those in other proteins (A and C).$

Immunoblot analyses of neutrophils from CGD patients, T.M. (A), T.H. (B), and J.O. (C). The membrane fractions from neutrophils (1 to 2 × 10⁶ cell equivalents) were electrophoresed and transferred to nitrocellulose membranes, which were treated with antiserum against polypeptides of p22-phox and gp91-phox and stained with Ponceau. (A) Patient T.M. (3), mother (2), and controls (1 and 4). (B) Patient T.H. (2) and controls (1 and 3). (C) Patient J.O. (2) and controls (1 and 3). The protein concentration of the variant CGD (B) loaded was higher than those in other proteins (A and C).

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