Aerolysin sensitivity correlates CD59 expression in PNH cell lines. Representative example showing correlation between aerolysin sensitivity and flow cytometric detection of CD59 in PNH cell lines. (A) Flow cytometric analysis for CD59 expression in a population of cells consisting of 90% LD⁻PIGA⁺ cells and 10% LD⁻ cells (solid line). LD⁻ cells are depicted by the dotted line. (B) The percentage of viability of the LD⁻ (solid line) and mixed cell (dotted line) populations after exposure to 1 nmol/L aerolysin at 37°C. Cell viability was determined by trypan blue exclusion at 5-minute intervals.