Restoration of GPI-anchor expression in PNH cell lines overcomes resistance to aerolysin. Flow cytometric analysis for CD59 expression of LD⁻ and JY5 cells before (solid line) and after (dotted line) stable transfection of the PIGA cDNA (A and C). (B) Viability of LD⁻ (solid line) and LD⁻PIGA⁺ (dotted line) cells after exposure to 1 nmol/L aerolysin at 37°C. (D) Viability of JY5 (solid line) and JY5PIGA⁺ (dotted line) cells after exposure to 1 nmol/L aerolysin at 37°C. Cell viability was determined in triplicate at 5-minute intervals. Error bars represent the standard deviations.