Expression of mOSMRβ mRNA. (A) Northern blot analyses of mOSMRβ mRNA in various cell lines. PolyA⁺ RNAs were prepared from various cell lines, and 1 μg of each sample was subjected to Northern blot analysis: lane 1, LO cells; lane 2, NIH3T3 cells; lane 3, Ba/F3 cells; lane 4, CCE cells; lane 5, M1 cells. (B) Northern blot analyses of mOSMRβ mRNA in various adult tissues; 1 μg of each sample was subjected to Northern blot analyses: lane 1, brain; lane 2, lung; lane 3, heart; lane 4, liver; lane 5, kidney; lane 6, small intestine; lane 7, muscle; lane 8, thymus; lane 9, spleen; lane 10, LO cells (upper panel). The middle panel shows the long exposure of the upper panel. The lower panel demonstrates equal loading by rehybridization with the GAPDH probe.