Apoptotic effects of CD437 on NB4 cells. NB4 cells (200,000/mL) were treated with vehicle alone (DMSO, Control), CD437 (10−6 mol/L), or Staurosporine (Stauro, 10−6 mol/L) for 16 hours. (A) Fluorescence microphotographs of DAPI-stained cells (original magnification × 40) are shown in the top two panels. The bottom panels show flow cytometric TUNEL analyses with forward scatter on the horizontal axis and fluorescence intensity on the vertical axis. The percentage of apoptotic, necrotic, and viable cells is indicated on the top-right, bottom-left, and bottom-right, respectively. (B) Intranucleosomal DNA fragmentation, as assessed by agarose gel electrophoresis. LMW, centrifugation-resistant low–molecular weight DNA; HMW, high–molecular weight DNA.
