FACS analysis of CXCR4, CCR5, and CCR2 on subpopulations of BMMNC. Total BMMNC were stained with anti-CXCR4, anti-CCR5, and anti-CCR2 antibodies as described and FACS analysis was performed on the gated populations as indicated in Fig 1A and D. R1, R2, and R3 represent the lymphocyte, granulocyte precursor, and monocyte gates, respectively. The isotype negative controls are overlaid (bold line), and M1 represents the positive populations. Data from at least 3 different donors were analyzed. The mean percentage of positive cells for each chemokine receptor plus or minus the standard deviation is summarized in Table 1. Histograms from a representative donor are presented.