Expression of chemokine receptors on total BMMNC. BMMNC were isolated from bone marrow aspirates of healthy donors by Ficoll-gradient centrifugation; stained with MoAbs to CXCR4 (B), CCR5 (C), CCR2 (E), and CCR3 (F); and subjected to FACS analysis as described in Materials and Methods. The histogram represents analysis of 10,000 events acquired in the total ungated population (A and D). The isotyped matched negative control is shown in the overlay. M1 gate represents the positive populations. Data from at least 3 different donors were analyzed with similar results. Data from a representative donor are presented.