RT-PCR analysis of the expression of PKC-ɛ and - mRNA in the subclones of the 32D cell lines. The ethidium bromide staining of products amplified by 30 cycles of PCR with primers specific for the PKC-ɛ and - or for murine actin and separated by electrophoresis on agarose gel are presented. Comparable amounts of products of the expected molecular weights were amplified not only from cDNA obtained from human cord blood (last lane) and mouse bone marrow (first lane) mononuclear cells, as controls, but also from cDNA obtained from all subclones of the 32D cell lines. The specificity of the PKC-ɛ band amplified from the 32D Epo cell line was proved by direct sequencing of the band eluted from the gel.