Fig. 5.
Fig. 5. Anti-B6dom1 CTLs expand more rapidly than anti–H-Y CTLs. Limiting numbers of responder spleen cells from C3H.SW female mice primed either with C57BL/6 female (B6dom1+) or C3H.SW male (H-Y+) splenocytes (20 × 106cells injected IP) were restimulated in vitro on days 5, 10, 15, or 20 postimmunization with 3 × 105 irradiated stimulator cells in culture medium supplemented with 2.5 U/mL of IL-2. After 9 days, cultures were evaluated in a 4-hour cytotoxicity release assay. Targets were T2Db cells coated with optimal concentrations of H-Y or B6dom1 peptide. Mean ± SD of three mice per group. *, Not detectable.

Anti-B6dom1 CTLs expand more rapidly than anti–H-Y CTLs. Limiting numbers of responder spleen cells from C3H.SW female mice primed either with C57BL/6 female (B6dom1+) or C3H.SW male (H-Y+) splenocytes (20 × 106cells injected IP) were restimulated in vitro on days 5, 10, 15, or 20 postimmunization with 3 × 105 irradiated stimulator cells in culture medium supplemented with 2.5 U/mL of IL-2. After 9 days, cultures were evaluated in a 4-hour cytotoxicity release assay. Targets were T2Db cells coated with optimal concentrations of H-Y or B6dom1 peptide. Mean ± SD of three mice per group. *, Not detectable.

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