Fig. 7.
![Fig. 7. Immunoprecipitation analysis of GPIIb-IIIa complexes from CHO cells transiently cotransfected with cDNAs encoding GPIIIa and either normal or [Arg674]GPIIb. CHO cells were transiently transfected with cDNAs encoding GPIIIa and either normal or [Arg674]GPIIb. Surface (upper panel) or total (surface and intracellular, lower panel) labeling of cells was performed with biotin-NHS and the GPIIb-IIIa complexes were immunoprecipitated with anti-GPIIIa (P37) or anti-GPIIb (M3) MoAbs and processed as described in Materials and Methods.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/93/3/10.1182_blood.v93.3.866/5/blod40311007w.jpeg?Expires=1724232802&Signature=X59~9tsdirc7GheXDsvhDPy~rY0x~5Olsr~aBTVYOwIk925JUQ4mMkJgQ0PScGytXcDmnVHYNS64TS31x6whSsjaUUTecRnSmcKLUzVp4QPhp6XzVWWEpgNNFsHd9nMCwMGmqIN15MCct2kQj8MzifDtR7A~2BhEtG2eEupE4WyB3EzJArmAHGrc6wN0lqJFV9Af-SY882HZh1qlt6e-zMSYPLJoqTUI0HJ61AgNiaiBzksmesj8RMdWIm3PsozwIh8bWuay2BYeGHkdIogWg3AdycpbT2s90vqvLeLexzvGKFUUwSZTqRD92v5pmRoKE3QikAWIG6YFt72f~r2jdg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Immunoprecipitation analysis of GPIIb-IIIa complexes from CHO cells transiently cotransfected with cDNAs encoding GPIIIa and either normal or [Arg674]GPIIb. CHO cells were transiently transfected with cDNAs encoding GPIIIa and either normal or [Arg674]GPIIb. Surface (upper panel) or total (surface and intracellular, lower panel) labeling of cells was performed with biotin-NHS and the GPIIb-IIIa complexes were immunoprecipitated with anti-GPIIIa (P37) or anti-GPIIb (M3) MoAbs and processed as described in Materials and Methods.
