Activity of ANUP on (A) 3 different KS cell lines and (B) control cell lines. All cell lines were plated in 48-well dishes at a density of 10,000/mL. The following day, a different concentration of ANUP was added in fresh media. Plates were incubated for an additional 4 days. Cells were stained with MTT and lysed, and the absorbance was read in an ELISA reader.