Fig. 3.
![Fig. 3. Detection of cell surface sialoadhesin lectin activity on Mm1 cells using a multivalent 3′-sialylactosylated probe (3′-PAA-FITC). Cultured Mm1 cells, a murine macrophage cell line, were washed, untreated, Arthrobacter Ureafaciens(AU)/VC-sialidase treated, or AU/VC-sialidase treated followed by preincubation with the 3D6 adhesion-blocking MoAb, and stained with FITC-conjugated 3′-PAA. Staining was detected using single color flow cytometry (fluorescence-activated cell sorting [FACS] analysis).](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/93/4/10.1182_blood.v93.4.1245/5/blod40409003x.jpeg?Expires=1724243336&Signature=W9oXb7HftC2OnFzllF6PO2db1QxW3NbaRWgCTXIkI1h~7OqTacaW7TaTyJ2bH3Gr-F9FoaUerDSVU1d6bW3MqeKIT7DQA2YkZkvjAz5LDGvVGITsTUeKztLWkZgYaCrvNIxJoZjpf8AFICcN2JVrqDYi9Et2Be6P7ZF5HY-iy2~Jg-5FxzcND4nxI-hRbJEuMjkHOEy6MYrrol4S7~uZITbYUconG4X3OLPaFDGDOp4cIe~Jzvs1cIw~kA40uZscTwbPgGloLLGnQ8I9z3Y7CSXH8sX6wT4MJBDN~7kuGSSieeUX6NKlxmGUd~VsUIS5FTa85SD4MFN6I5hTbwO8TQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Detection of cell surface sialoadhesin lectin activity on Mm1 cells using a multivalent 3′-sialylactosylated probe (3′-PAA-FITC). Cultured Mm1 cells, a murine macrophage cell line, were washed, untreated, Arthrobacter Ureafaciens(AU)/VC-sialidase treated, or AU/VC-sialidase treated followed by preincubation with the 3D6 adhesion-blocking MoAb, and stained with FITC-conjugated 3′-PAA. Staining was detected using single color flow cytometry (fluorescence-activated cell sorting [FACS] analysis).
