Fig. 6.
![Fig. 6. Radiosequencing of PR3 secreted from RBL/PR3 transfectant cells during a 6-hour incubation in the presence of [3H]-isoleucine. PR3 in the CM was immunoprecipitated and isolated on SDS-PAGE and transferred by Western blot to a PVDF membrane from which the radioactive band at 35 kD was excised and subjected to amino acid sequencing. The columns show the radioactivity of the first 10 cycles of sequencing, corresponding to the first 10 amino acids. Blank (bl) shows the background activity.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/93/3/10.1182_blood.v93.3.849/5/blod40307006x.jpeg?Expires=1724158703&Signature=s4bUH~Q4tLp2wUv3I6UGy9tcE7Osr23yl3IR9A2chwi3Q1t8gsLjBa8zHuo8zwt8~ufh7gnj5QvAwfd7wgjbNV0dKoBAwI1HpIiH119eb7b3Z7kSR-W3C3qcSJ087WtwCToN4G0yRiTf~WLb5S01X97g8qtRTrfpdIZ01lj36ofK9YUQ8zM49VCr8uGLw~bIQZk-IzJnsrQOrFQ2A0Yef7z7ym-tOnQkm3YBTFeuuf-gYU9cSpdT1Lmrv7viuU9VERWJ1vO~ElDpXtJHeeV46PNM6wNISFd8fpcYRq72HXMPNm0eZHgMx4Zs0g-XCwiTiHVvZdUlNzP9HAWdunCtuQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Radiosequencing of PR3 secreted from RBL/PR3 transfectant cells during a 6-hour incubation in the presence of [3H]-isoleucine. PR3 in the CM was immunoprecipitated and isolated on SDS-PAGE and transferred by Western blot to a PVDF membrane from which the radioactive band at 35 kD was excised and subjected to amino acid sequencing. The columns show the radioactivity of the first 10 cycles of sequencing, corresponding to the first 10 amino acids. Blank (bl) shows the background activity.
