TGF-β1 inhibited DC maturation from CD11b^+hiCD11c⁺, but not CD11b^−/dullCD11c⁺ DC precursors in the presence of GM-CSF + TNF. (A) Histograms of quad of Ia⁺CD86⁺ mature DCs were set up on the isotype-matched control dot plot. (B and C) The capacity of cultured cells to stimulate allogenic MLR. The experiments are representative of more than three independent experiments.