Expression of cytolytic mediators in HO-1. Expression of mRNAs for perforin, granzyme B, Fas ligand, TNF-, and LT-β was investigated by RT-PCR as detailed in Materials and Methods. mRNAs were extracted from HO-1, which had been stimulated with the DEK-CAN peptide and autologous APCs for 5 days (lane 1) and for 14 days (lane 2) and from PBMCs, which had been stimulated with phytohemagglutinin for 3 days (lane 3). Lane M shows 100-bp ladder markers.