Morphology of freshly isolated and cultured AML cells. Phase contrast micrographs of freshly isolated AML PBMNC (A; 100× origonal magnification); AML cells cultured for 14 days in GM-CSF, IL-4, and TNF (B; 250× original magnification); or GM-CSF, IL-4, and CD40L (C; 250× origianl magnification). (A and B) Obtained with cells from patient 13; (C) Obtained with cells from patient 18.